diff --git a/xarray/backends/zarr.py b/xarray/backends/zarr.py
index fe004c212b6..5d6425b37a0 100644
--- a/xarray/backends/zarr.py
+++ b/xarray/backends/zarr.py
@@ -279,7 +279,7 @@ async def async_getitem(self, key):
         )
 
 
-def _determine_zarr_chunks(enc_chunks, var_chunks, ndim, name):
+def _determine_zarr_chunks(enc_chunks, var_chunks, ndim, name, zarr_format):
     """
     Given encoding chunks (possibly None or []) and variable chunks
     (possibly None or []).
@@ -301,15 +301,18 @@ def _determine_zarr_chunks(enc_chunks, var_chunks, ndim, name):
     # while dask chunks can be variable sized
     # https://dask.pydata.org/en/latest/array-design.html#chunks
     if var_chunks and not enc_chunks:
+        if zarr_format == 3:
+            return tuple(var_chunks)
+
         if any(len(set(chunks[:-1])) > 1 for chunks in var_chunks):
             raise ValueError(
-                "Zarr requires uniform chunk sizes except for final chunk. "
+                "Zarr v2 requires uniform chunk sizes except for final chunk. "
                 f"Variable named {name!r} has incompatible dask chunks: {var_chunks!r}. "
                 "Consider rechunking using `chunk()`."
             )
         if any((chunks[0] < chunks[-1]) for chunks in var_chunks):
             raise ValueError(
-                "Final chunk of Zarr array must be the same size or smaller "
+                "Final chunk of a Zarr v2 array must be the same size or smaller "
                 f"than the first. Variable named {name!r} has incompatible Dask chunks {var_chunks!r}."
                 "Consider either rechunking using `chunk()` or instead deleting "
                 "or modifying `encoding['chunks']`."
@@ -475,6 +478,7 @@ def extract_zarr_variable_encoding(
         var_chunks=variable.chunks,
         ndim=variable.ndim,
         name=name,
+        zarr_format=zarr_format,
     )
     if _zarr_v3() and chunks is None:
         chunks = "auto"
@@ -853,9 +857,18 @@ def open_store_variable(self, name):
         )
         attributes = dict(attributes)
 
+        if hasattr(zarr_array, "metadata"):
+            chunk_grid = zarr_array.metadata.chunk_grid
+            chunks = getattr(chunk_grid, "chunk_shapes", None)
+            # regular chunk grid
+            if chunks is None:
+                chunks = chunk_grid.chunk_shape
+        else:
+            chunks = zarr_array.chunks
+
         encoding = {
-            "chunks": zarr_array.chunks,
-            "preferred_chunks": dict(zip(dimensions, zarr_array.chunks, strict=True)),
+            "chunks": chunks,
+            "preferred_chunks": dict(zip(dimensions, chunks, strict=True)),
         }
 
         if _zarr_v3():